Genome-wide analysis of DNA methylation identifies novel cancer-related genes in hepatocellular carcinoma

Masahiro Shitani, Shigeru Sasaki, Noriyuki Akutsu, Hideyasu Takagi, Hiromu Suzuki, Masanori Nojima, Hiroyuki Yamamoto, Takashi Tokino, Koichi Hirata, Kohzoh Imai, Minoru Toyota, Yasuhisa Shinomura
Tumour Biol. Oct 11, 2012
… Total RNA was extracted using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) and then treated with a DNA-free kit (Ambion, Austin, TX, USA). Genomic DNA and total RNA from normal liver tissue from a healthy individual were purchased fromBioChain (Hayward, CA, USA). …

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