Frequent epigenetic inactivation of DICKKOPF family genes in human gastrointestinal tumors

Hironobu Sato, Hiromu Suzuki, Minoru Toyota, Masanori Nojima, Reo Maruyama, Shigeru Sasaki, Hideyasu Takagi, Yohei Sogabe, Yasushi Sasaki, Masashi Idogawa, Tomoko Sonoda, Mitsuru Mori, Kohzoh Imai, Takashi Tokino, Yasuhisa Shinomura
Carcinogenesis Dec 1, 2007
… …treated with a DNA-free kit (Ambion, Austin, TX). Total RNA from normal colon mucosa from a healthy individual was purchased from BioChain (Hayward, CA). Reverse transcriptase-polymerase chain reaction Single-stranded cDNA was prepared using SuperScript III… …

Leave a Reply