Expression profiles of different cytokine genes in peripheral blood mononuclear cells of goats infected experimentally with native strain of Mycobacterium avium subsp …

Singh, Singh, Saxena, Singh
Animal Jan 0, 1900
… RNA samples were stored at -80°C, until use. First strand cDNA synthesis. cDNA was prepared from total extracted RNA using oligo (dt) 18 primer and MMLV transcriptase according to the manufacturer’s instructions (BioChain Institute, Inc. USA). …

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