Expression and Regulation of 11-β Hydroxysteroid Dehydrogenase Type 2 Enzyme Activity in the Glucocorticoid-Sensitive CEM-C7 Human Leukemic Cell …

Schmidt
ISRN oncology Jan 0, 1900
… Total homogenate protein was isolated by homogenization of CEM-C7 cells, adult human liver tissue, or adult human kidney tissue (BioChain Institute, Hayward, CA) in lysis buffer (1 nM phenylmethylsulfonyl fluoride in PBS), followed by centrifugation (10 minutes at 600 ×g …

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