Enhanced mineralization potential of vascular cells from SM22α-Rankl (tg) mice

S Morony, A P Sage, T Corbin, J Lu, Y Tintut, L L Demer
Calcif. Tissue Int. Dec 8, 2012
… Gene Expression Total RNA was isolated from cells using TRIzol reagent (Invitrogen Carlsbad, CA). Real-time RT-qPCR was per- formed using a one-step method (BioChain Institute, Hay- ward, CA) in the Mx3005P (Stratagene, La Jolla, CA). b-Actin was used for normalization. …

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