Early mechanisms of pathobiology are revealed by transcriptional temporal dynamics in hippocampal CA1 neurons of prion infected mice

Anna Majer, Sarah J Medina, Yulian Niu, Bernard Abrenica, Kathy J Manguiat, Kathy L Frost, Clark S Philipson, Debra L Sorensen, Stephanie A Booth
PLoS Pathog. Nov 12, 2012
… Pre-hybridization of slides was performed by exposing the slides to the pre-hybridization buffer (BioChain Institute, Inc.) for 4 hours at 60°C. We added 50 nM of the linearized DIG-labeled miRNA-132 LNA probe (Exiqon) in ready to use hybridization solution (BioChain Institute …

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