DNA methylation of developmental genes in pediatric medulloblastomas identified by denaturation analysis of methylation differences

Scott J. Diede, Jamie Guenthoer, Linda N. Geng, Sarah E. Mahoney, Michael Marotta, James M. Olson, Hisashi Tanaka, Stephen J. Tapscott
PNAS Dec 1, 2009
… …manufacturer’s pro- tocol (Invitrogen) and quantitated using a NanoDrop spec- trophotometer. Normal cerebellum RNA was obtained from Biochain. First-strand cDNA synthesis was performed using random hexamers on 1 g of total RNA as input, using the Su- perScript III… …

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