Diversity in Tissue Expression, Substrate Binding, and SCF Complex Formation for a Lectin Family of Ubiquitin Ligases

Kevin A. Glenn, Rick F. Nelson, Hsiang M. Wen, Adam J. Mallinger, Henry L. Paulson
J. Biol. Chem. May 1, 2008
… …and pelleting debris by centrifugation at 16,000 g, 4 C for 15 min. Mouse tissue preparations from a commercial supplier (BioChain, Hayward, CA) produced similar results. Immunoprecipitation (IP) Nondenatured lysates from 10-cm plates were incubated… …

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