Differential modulation of 3T3-L1 adipogenesis mediated by 11-hydroxysteroid dehydrogenase-1 levels

Jaime Kim, Karla A. Temple, Sara A. Jones, Kimberly N. Meredith, Juliana L. Basko, Matthew J. Brady
J. Biol. Chem. Feb 1, 2007
… …TAC AAA CAT. Replicate PCR reactions (50 l final volume) were run using 2.5 ng of murine hepatic or adipocytic cDNA library (Biochain Institute Inc, Hayward, CA), 100 pmol of each primer and 400 M dNTPs in 60 mM Tris (pH 9.0)/15 mM ammonium sulfate/2 mM MgCl2… …

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