Development of a novel ectonucleotidase assay suitable for high-throughput screening

Kris F Sachsenmeier, Carl Hay, Erin Brand, Lori Clarke, Kim Rosenthal, Sandrine Guillard, Steven Rust, Ralph Minter, Robert Hollingsworth
J Biomol Screen Aug 12, 2012
… Human NT5E (NM_002526), minus the GPI linkage region, was obtained from placenta cDNA (BioChain, Newark, CA) using standard RT PCR and cloned into a mammalian expression vector (amino acids 1–552 cloned; GPI linkage region from amino acids 556–572 was not …

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