DACT1, an antagonist to Wnt/β-catenin signaling, suppresses tumor cell growth and is frequently silenced in breast cancer
X Yin, T Xiang, L Li, X Su, X Shu, X Luo, J Huang, Y Yuan, W Peng, M Oberst, K Kelly, G Ren, Q Tao.
Breast Cancer Res. Mar 12, 2013
…Total RNA, including the total RNA from 4 normal pituitary tissues (BioChain Institute, Hayward, CA, USA) as a positive control, was reverse-transcribed using M-MLV transcriptase (Invitrogen, Carlsbad, CA, USA) with oligo(dT)12-18 primers (Invitrogen). … penicillin, and streptomycin. Normal human breast tissue RNA samples were purchased commercially (Stratagene, La Jolla, CA, USA; Millipore Chemicon, Billerica, MA, USA; BioChain Institute, Hayward, CA, USA). Primary breast …