Crystal structure of the catalytic domain of a human thioredoxin-like protein: Implications for substrate specificity and a novel regulation mechanism

Jian Jin, Xuehui Chen, Yan Zhou, Mark Bartlam, Qing Guo, Yiwei Liu, Yixin Sun, Yu Gao, Sheng Ye, Guangtao Li, Zihe Rao, Boqin Qiang, Jiangang Yuan
Eur. J. Biochem. Apr 1, 2002
… DDRT-PCR and full-length cDNA isolation Total RNA (2.5 µg) from 13- and 33-week-old human fetal cerebrum (Biochain) was reverse transcribed by Superscript II (Gibco-BRL) using a single-base anchored 3′ primer (5′-AAGCTTTTTTTTTTTN-3′, N=C, G, …_x000D_
… Northern blot analysis Total Poly(A) + RNA from 13- and 33-week-old human fetal cerebrum (Biochain) was electrophoresed in a 1% agarose gel containing 0.66 M formaldehyde and was blotted onto a …

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