Computational analysis of bacterial RNA-Seq data

Ryan McClure, Divya Balasubramanian, Yan Sun, Maksym Bobrovskyy, Paul Sumby, Caroline A Genco, Carin K Vanderpool, Brian Tjaden
Nucleic Acids Res. May 28, 2013
… A complementary DNA (cDNA) library of the resulting messenger RNA (mRNA) was then prepared using BioChain‘s Directional mRNA Sample Prep kit according to the manufacturer’s instructions. Briefly, RNA was fragmented with metal ion scission and treated with PNK. …

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