Characterization of PCEng2, a β-1,3-Endoglucanase Homolog in Pneumocystis carinii with Activity in Cell Wall Regulatio

Leah R. Villegas, Theodore J. Kottom, Andrew H. Limper
Am. J. Respir. Cell Mol. Biol. Aug 1, 2010
… …enzyme (HindIII or EcoRI; Promega, Inc., Madison, WI) for 4 hours at 37C. Genomic DNA (10 mug) from normal rat lung tissue (BioChain, Hayward, CA) was also digested under the same conditions as controls. The digested genomic DNA was then electrophoresed through… …

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