Characterization of a novel splice variant of δ ENaC subunit in human lungs

Run-Zhen Zhao, Hong-Guang Nie, Xue-Feng Su, Dong-Yun Han, Andrew Lee, Yao Huang, Yongchang Chang, Sadis Matalon, Hong-Long Ji
Am. J. Physiol. Lung Cell Mol. Physiol. Jun 15, 2012
… Following two washes with DEPC-PBS, slides were treated with Proteinase K (100 µg/ml) at 37°C for 15 min. Slides were rinsed once with DEPC-water and prehybridized at 42°C for 2 h in prehybridization solution (IsHyb ISH kit, BioChain Institute, Hayward, CA). …

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