c-Jun N-Terminal Kinase Activation Responses Induced by Hippocampal Kindling Are Mediated by Reactive Astrocytes

Kasie K. Cole-Edwards, Alberto E. Musto, Nicolas G. Bazan
J. Neurosci. Aug 1, 2006
… …Alternatively, to separate brain tissue into cytosolic, membrane, and nuclear protein fractions, a compartmental extraction kit from BioChain (Hayward, CA) was used. Tissue was homogenized in a Dounce homogenizer with five strokes of the loose-fitting pestle and manipulated… …

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