Angiotensin II-induced mitochondrial Nox4 is a major endogenous source of oxidative stress in kidney tubular cells

Su-Mi Kim, Yang-Gyun Kim, Kyung-Hwan Jeong, Sang-Ho Lee, Tae-Won Lee, Chun-Gyoo Ihm, Ju-Young Moon
PLoS ONE Jul 18, 2012
… Isolation of Mitochondria (Mt). NRK-52E cells were harvested from cultures and fractionated into cytosol (Cyt) and mitochondria using a mitochondrial isolation kit (BioChain Institute, Inc., CA, USA) according to the manufacturer’s instructions. …

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