Angiopoietin-like 4 (Angptl4) protein is a physiological mediator of intracellular lipolysis in murine adipocytes

Nora E Gray, Lily N Lam, Karen Yang, Anna Y Zhou, Suneil Koliwad, Jen-Chywan Wang
J. Biol. Chem Mar 9, 2012
… Reverse transcription was performed as described (6). The resulting cDNA was diluted to 170 µl, and 3.5 µl was used to perform qPCR in a 25-µl reaction using the EVA qPCR SuperMix kit (BioChain) per the manufacturer’s protocol. …

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