Analysis of osteopontin levels for the identification of asymptomatic patients with calcific aortic valve disease

Juan B Grau, Paolo Poggio, Rachana Sainger, William J Vernick, William F Seefried, Emanuela Branchetti, Benjamin C Field, Joseph E Bavaria, Michael A Acker, Giovanni Ferrari
Ann. Thorac. Surg. Jan 21, 2012
… In situ mRNA hybridization was used to detect total OPN and its isoforms with the custom-designed probes purchased from Exiqon (Woburn, MA). An in situ hybridization kit from BioChain Institute Inc (Hayward, CA) was used to perform the detection steps. In Vitro Calcification. …

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