Alternatively spliced variants of the regucalcin gene in various human normal and tumor tissues

Tomiyasu Murata,Masayoshi Yamaguchi
International Journal of Molecular Medicine: Spandidos Publications Jul 18, 2014
The human cDNA and protein samples, which were prepared from normal (non-tumor) and tumor tissues, were obtained from BioChain Institute. Total RNA in the non-tumor (normal) and tumor tissues from human liver, kidney, brain and lung tissues (BioChain Institute) was isolated using the guanidine thiocyanate technique. Total protein lysates, which were obtained from normal and tumor tissues of human liver and kidney (BioChain Institute), were used for western blot analysis

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