Alternative splicing and proteolytic rupture contribute to the generation of soluble IL-6 receptors (sIL-6R) in rheumatoid arthritis

José Ramón Lamas, Luis Rodríguez-Rodríguez, Pilar Tornero-Esteban, Esther Villafuertes, José Hoyas, Lydia Abasolo, Jezabel Varadé, Roberto Alvarez-Lafuente, Elena Urcelay, Benjamín Fernández-Gutiérrez
Cytokine Mar 11, 2013
… for the experiments. Total RNA was obtained from PBMCs using an RNeasy Mini Isolation Kit (Qiagen). RNA (10 ng) was retrotranscribed, using BioChain‘s Optimax First Strand cDNA Synthesis Kit (BioChain). RT-PCR was …

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