Stefan Nagel, Corinna Meyer, Maren Kaufmann, Margarete Zaborski, Roderick A. F. MacLeod, Hans G. Drexler
PLOS One May 10, 2018
Using TRIzol reagent (Invitrogen, Karlsruhe, Germany) we extracted total RNA from cell lines. Total RNA from primary human cells and tissues was commercially obtained. We used peripheral mononuclear blood cells (PMBC), CD3-positive T-cells and CD19-positive B-cells (Miltenyi Biotec, Bergisch Gladbach, Germany), retina, spleen and thymus (Biochain, Newark, CA, USA).