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Membrane proteins are an integral part of molecular biology research. These proteins interact with biological membranes and are the target of over 50% of all modern medicinal drugs. Membrane proteins perform a variety of functions...(Expand Details) that are crucial to the survival of organisms such as relaying signals, transport, and cell adhesion. Additionally, approximately 20 – 30 % of genes within a genome encode membrane proteins.

At BioChain, we’ve prepared membrane proteins from a large variety of documented quality tissues that are ready-to-use for genetic testing and analysis. Our tissue membranes are prepared from whole tissue homogenates and are of high-quality. Distinction of quality is ensured by consistent patterns on SDS-PAGE analysis, as well as by storage in protease inhibitor cocktail buffer.

Features


  • Membrane protein isolated from diseased and normal tissues and from tumor cell lines

  • Available from human, monkey and rat tissues

  • Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA

  • Extensive quality control procedures to ensure high quality

  • Documentation of tissues' clinical history is available


Applications

 

  • Western Blot (10 µg to 20 µg per lane is recommended for a mini gel)

  • Immunoprecipitation

  • Electrophoresis

  • Enzymatic activity analysis

  • Protein-protein interaction

  • Tissue specific expression


Quality Control

 

  • Protein lysate is verified on SDS-PAGE analysis as a consistent pattern.

  • Protein lysate is positively confirmed by Western Blot using specific biomarker: GAPDH for total protein, cytoplasmic and universal total protein; Histone for nuclear protein; Na/K ATPase for membrane protein; Vimentin for cytoskeleton protein.

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