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cfPure® DNA Free Plasma

Features of cfPure® DNA Free Plasma

The extraction steps of a cfDNA-based assay may contribute to significant variation in the final result due to factors in human plasma that affect either the extraction or the subsequent PCR. One reason for the lack of research in this area is that contamination of gDNA from normal plasma donors prevents accurate cfDNA (~170bp) measurement. BioChain offers cfPure DNA-free plasma which has all the DNA removed using proprietary technology without affecting the composition of other plasma components. With this product, the DNA content is as low as 0 to 10 pg/ml. Other plasma components such as total protein, immunoglobulins, and lipids are all within the normal range. This DNA-depleted plasma may be used as a matrix for QC standards, focusing on its ability to monitor both the specificity and sensitivity of the cfDNA extraction method.

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cfPure® DNA Free Plasma

Features

  • Ideal control reagent for cfDNA assay validation
  • Better mimics patient samples than artificial matrices
  • Better stability than artificial matrices
  • Known pathogen (HIV, HBV, HCV, HTLV, Syphilis) free
  • Ready-to-use

Plasma integrity check:

  • DNA: < 4 pg/ml
  • Total protein: 5-7 g/dL
  • Immunoglobulin G: 500-1700 mg/dL
  • Immunoglobulin A: 90-400 mg/dL
  • Immunoglobulin M: 20-172 mg/dL
  • Cholesterol, Total: 50-199 mg/dL
  • Triglycerides: 50-149 mg/dL
  • HDL Cholesterol: > 20 mg/dL
  • VLDL Cholesterol: 10-40 mg/dL
  • LDL Cholesterol: 20-99 mg/dL
  • pH: 7.2-7.6
  • Negative for HIV, HBV, HCV,
  • HTLV and Syphilis.

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