Protein Extraction Kits

One of the challenges of functional proteomics is separation of complex proteins for quantitative and differential localization analysis. This necessitates standardized and repeatable operation procedures to isolate protein from tissues and cells. BioChain is the leading provider of protein lysates extracted from hundreds of different tissues and cells. We also offer simple and reliable protein extraction kits that provide tools for protein lysate extraction. The kits we provide are high quality and enable efficient and reproducible results. Extract total protein, compartmental proteins, and membrane proteins with these 4 unique kits. Bulk quantities and OEM manufacturing are also available for purchase at highly competitive prices.

Features

  • Provides all components for stepwise preparation of proteins from tissues and cells
  • Isolate compartmental proteins, membrane proteins, or total protein
  • Increase detection sensitivity by enriching a subset of proteins of interest
  • Easy to use compared to other methods such as differential centrifugations

Applications

  • Prepare proteins for downstream applications such as SDS-PAGE, Western blotting, gel mobility shift assay, protein assays, protein-protein interaction, and more.
  • Detection of differential post-translational modifications or differential subcellular localization of target proteins
  • Enrichment of low-abundance proteins for visualization and subsequent analysis
  • Membrane protein expression and receptor function study
  • Use to study: DNA binding proteins such as transcription factors, soluble proteins abundant in cytosol, membrane proteins such as receptors, and cytoskeletal proteins

Quality Control:

Our kits are tested by going through the complete protein extraction process. For example, our Compartmental Protein Extraction kit has been tested to go through the complete compartmental protein extraction procedure from rat colon. Four compartmental proteins from the extraction are used for electrophoresis, transferring to PVDF membrane and immunoblotting with mouse anti-GAPDH, anti-Histone H1, anti-Vimentin, and Na/K+ ATPase as primary antibodies, and HRP-conjugated anti-Mouse IgG as secondary antibody. The separation of four compartment proteins is confirmed by the enrichment of GAPDH in the cytoplasmic fraction, Histone H1 in the nuclear fraction, Na/K+ ATPase in the membrane fraction, and the unique localization of Vimentin in the cytoskeleton fraction.
Learn More
Heading
Cytoplasmic Fraction
Yes
Yes
No
N/A
Nuclear Fraction
Yes
Yes
No
N/A
Membrane Fraction
Yes
Yes
Yes
N/A
Cytoskeletal Fraction
Yes
No
No
N/A
Total Protein (cytoplasmic, nucleus, membrane, and cytoskeletal proteins)
N/A
N/A
N/A
Yes
Shop By

16 Results

Set Descending Direction
  1. 1 kit
    K3012010
    $377.00
  2. 22 ml
    K3014005-1
    $73.00
  3. 6 ml
    K3014005-2
    $67.00
  4. 13 ml
    K3011010-1
    $117.00
  5. 260 ┬Ál
    K3011010-2
    $249.00
  6. 1 kit
    K3011010
    $334.00
  7. 1 kit
    K3013010
    $465.00
Page
per page

Need biospecimens from multiple donors?

Reach out to us to customize your project! We offer sample collection + preparation, extraction & purification, and sample analysis services.