Universal cDNA Reverse Transcribed by Oligo dT Primer: Mouse Normal Tissues
BioChains’ Mouse Universal cDNA is prepared from several male and female Balb/C mice whole bodies without fur. This cDNA serves as a standard for comparison of gene expression by RT-PCR or as a gene pool for cloning mouse genes
BioChain’s universal cDNA is prepared using the following method. 10 Âµg total RNA was primed by an oligo dT primer or random hexamer through reverse transcription (RT) using MMLV reverse transcriptase in 40 Âµl final volume. The RT Reaction was stopped by heating at 65Â°C for 10 minutes. The resulting cDNA is contained in 1x RT buffer (1x RT Buffer: 50 mM Tris-CI, pH 8.3, 75 mM KCI, 3 mM MgCI2, 10 mM DTT). The estimated cDNA concentration is 2.5 ng/Âµl. 1 Âµl cDNA is enough for one PCR reaction.
No manuals for this product.
- Male: 5-6 wks old, 5 donors/Female: 5-6 wks old, 16 donors
- 100 rxn