QMTT Cell Viability Assay Kit

Overview

BioChain’s QMTTassay kit provides a convenient, sensitive, quantitative and reliable assay for determining the number of viable cells in a given culture. This homogeneous colorimetric assay is based on the conversion of a tetrazolium salt MTT, a pale yellow substrate, to formazan, a purple dye. This cellular reduction reaction involves the pyridine nucleotide cofactors NADH/NADPH and is only catalyzed by living cells. The formazan product has a low aqueous solubility and is present as purple crystals. Dissolving the resulting formazan with a solubilization buffer permits the convenient quantification of product formation. The intensity of the product color, measured at 550 – 620 nm, is directly proportional to the number of living cells in the culture. Reagents in the kit have been carefully formulated and optimized for sensitivity, assay robustness and automation.

Description

BioChain’s Cell-Based Assay Kits address the pharmaceutical and biotechnology customers’ ever-growing needs driven by the deluge of drug targets derived from genomics and proteomics. Our customers are seeking to perform large scale screens in cell-based systems because of the accurate and whole cell data which are promising and representative of the organism’s physiological condition. The kit comes with a complete system of all reagents for the assay and an easy-to-follow protocol.