M-MLV Reverse Transcriptase
BioChain provides a series of popular used modifying enzymes and polymerases for molecular biology
M-MLV reverse transcriptase is an RNA-dependent DNA polymerase. This enzyme is used to synthesize a complementary DNA strand from single-stranded RNA and DNA. Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase lacks DNA endonuclease activity, and has lower RNase H activity than the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase. BioChain also carries a newly engineered Ultrascript Reverse Transcriptase which is a more efficient version of M-MLV reverse transcriptase. It has minimum RNase H activity and maximum thermal stability. This enzyme is purified to near homogeneity to ensure high thermal stability, high specificity, high fidelity and more full length cDNA synthesis. With this enzyme, first-strand cDNA synthesis is optimal at 55oC, and it has a broad working temperature range of 37oC to 60oC. Typical yield for cDNA product size is 100 bp to 12 kb.
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- 100,000 units