Hotstart Taq DNA Polymerase

Overview

BioChain’s Taq Polymerase polymerizes DNA from a primer annealed to a DNA template in the presence of deoxyribonucleotide triphosphates. It works excellent for not only single PCR but also for multiplex PCR. BioChain’s Taq Polymerase is highly purified using a proprietary procedure that virtually eliminates contaminating bacterial DNA and provides excellent reproducibility between lots.

Description

BioChain’s HotStart Taq Polymerase combines high specificity, sensitivity, and minimal optimization with a fast 10-minute activation time. During PCR reaction setup, DNA polymerases commonly exhibit some synthesis activity which can lead to amplification of mis-primed sequences and accumulation of primer-dimers that compromise the specificity and yield of the reaction. Non-specific amplification can be minimized with HotStart Taq Polymerase. Our ready-to-use HotStart Taq Polymerase is chemically modified causing its activity to be blocked at lower temperatures. Activity is restored when the enzyme is heated to 95oC during the initial denaturing process, enabling hot-start PCR. BioChain’s Hot-Start Taq Polymerase is a powerful, yet cost effective way to minimize pre-cycling polymerase activity for more efficient, specific PCR.

SDS
Certificate of Analysis

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  • L7052001
  • 1000 units