Cholesterol Assay Kit

Overview

BioChain’s cholesterol assay kit is a simple, direct and automation-ready assay based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene-3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.

Description

BioChain’s BioAssay kits are for both routine laboratory tests and high-throughput drug screening applications. These kits use harmless non-radioactive assay formats such as fluorescence, bio- and chemiluminescence detection techniques. With our assay kits, researchers need little to no time for assay optimization.