- 1 Kit (100 ml)
BioChain’s cfPure™ Cell Free DNA Purification Kit is designed for rapid and efficient isolation of circulating cell free DNA (cfDNA).
BioChain’s cfPure® Cell Free DNA Extraction Kit is designed for rapid and efficient purification of circulating cell free DNA (cfDNA). The system utilizes silica-coated paramagnetic particles to purify cell free DNA from less than 1 mL to greater than 10 mL of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp – 500 bp DNA fragments in order to maximize recovery of cell free DNA. The recovered DNA is suitable for a wide range of down-stream applications, such as bisulfite sequencing, NGS, and qPCR.
Cfpure is designed to be a scalable kit. This means that you can use just the amount of beads and reagents that you need for your cfDNA extraction. This means that the number of extractions per kit is dependent on the amount of sample you wish to purify DNA from. For example, if you are working with 1mL samples, our 100mL kit (Catalog # K5011610) can process up to 100 samples, and our 250 mL kit (Catalog # K5011610) can process 250 samples. If you are working with 10mL samples, our 100mL kit (Catalog # K5011610) can process up to 10 samples, and our 250 mL cell free DNA Extraction kit (Catalog # K5011625) can process 25 samples.”
Cost Effective: <$3 per extraction from 1 mL plasma (List price for 250mL kit).
Automation Friendly: We’re experienced at automating cfPure® on both the KingFisher™ and on pipette-based instruments and takes approximately 50 minutes.
Versatile: Paramagnetic bead based system is amenable to extraction from <1mL to >10mL serum or plasma – in single tubes or 24- to 96-well plates.
Reproducible: Our beads, chemistry, and protocols are optimized for consistent results.
Applications: Extracted cell free DNA is suitable for NGS, qPCR and other demanding applications.
Time Efficient: Per extraction protocol takes less than 75 minutes.
The cfPure® protocol is rapid and easy, allowing users to process several samples in an hour or less and making it ideal for biomarker screening. BioChain has developed its own silica-coated magnetic bead technology, which is designed to efficiently recover low molecular weight cell free DNA during isolation. In addition to being easy to perform, and easy to automate, you can use less magnetic bead solution if you are purifying small amounts of plasma – saving you money.
Cell free DNA offers a library of information for the non-invasive detection of cancer and prenatal genetic testing, find out more. cfDNA is present in the human blood at varying levels, from 1 to 100 ng per ml of plasma. The size of cell free DNA is typically 100 bp to 500 bp in length (with most fragments being about 160-180 bp long), making traditional DNA extraction difficult. The key to successful analysis of cfDNA is to ensure efficient purification of smaller DNA fragments (100-500 bp). As Figure 1 shows, the cfPure® Cell Free DNA Extraction Kit does efficiently recover these important small fragments. Lastly, while cfPure® employs a magnetic bead-based DNA extraction method, the recovered cell free DNA performs identically in sensitive applications, such as Next Generation Sequencing, to current spin column based cfDNA kits on the market.
Our optimized magnetic bead based protocol enables the user to scale the protocol for use with any sample volume from 100 µl to 10 ml with highly reproducible results (Table 1). We also offer two kit sizes to fit your needs. Our 100 ml cell free DNA extraction kit contains enough reagents to extract cfDNA from 100 ml of human plasma. Our larger 250 ml cell free DNA extraction kit contains sufficient reagents to extract cfDNA from 250 ml of human plasma.