cDNA – Alzheimer’s Disease: Brain: Hippocampus
BioChain’s PCR Ready First Strand cDNAs are available from human neurological diseased brain tissues. 1 Âµl cDNA is sufficient for each PCR reaction.
First-Strand cDNA is synthesized from RNA isolated from a wide variety of documented human adult and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey and plant tissues. Total RNA used for cDNA synthesis is isolated by modified guanidine thiocyanate techniques. 10 Âµg total RNA was primed by an oligo dT primer and reverse transcription (RT) using MMLV reverse transcriptase in 40 Âµl final volume. RT reaction was stopped by heating at 65Â°C for 10 minutes. The cDNA is delivered in 1x RT buffer (1x RT Buffer: 50 mM Tris-Cl, pH 8.3, 75 mM KCl, 3 mM MgCl2, 10 mM DTT). 1 Âµl cDNA is sufficient for one PCR reaction. The 5′ end of human clathrin cDNA (a 6 kb gene) has been amplified by PCR from all of these cDNAs.