Ribonucleic acid (RNA) is vital to life, being present everywhere – from carrying genetic information to the production of deoxyribonucleic acid (DNA). However, RNA is one of the most fragile and delicate samples to extract and must be handled with care due to the presence of ribonuclease enzumes (RNases) which quickly degrade the RNA upon contact. RNases are present nearly everywhere, such as in water, skin, and even sometimes the researcher’s breath. Therefore, it is of absolute importance to use special care during RNA extraction and isolation. Detailed below is a useful method of RNA extraction from frozen tissues.
Figure 1 Right: Ribonucleic Acid (RNA) in the Nucleus Source