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mRNA Sample Prep Kit

Introduction to mRNA Sample Prep Kit

RapidSeq Directional mRNA Sample Prep Kit 1Transcriptome sequencing or RNA-seq is a novel method for gene expression analysis. Advantages of RNA-Seq include no bias toward known RNA molecules as with probe-based technologies, ability to detect novel and rare transcripts or novel alternative splice isoforms, and direct measurement of transcript abundance within biological samples. BioChain's RapidSeq Sample Prep Kits provide high quality solutions to the RNA-Seq Next Generation sequencing research and diagnostic communities. The RapidSeq Directional mRNA Sample Prep kit aims to prepare directional NGS libraries for subsequent cluster generation, using purified mRNA as starting material. Our kit provides strand-specific cDNA synthesis with reduced costs and increased sensitivity. Strand-specific RNA-seq improves on standard RNA-seq in three ways: accurately identifying antisense transcripts, determining the transcribed strand of non-coding RNAs (e.g. lincRNAs), and demarcating the boundaries of closely situated or overlapping genes. The protocol includes steps for mRNA fragmentation, purification, adapter ligation, reverse transcription, PCR amplification, and DNA fragment enrichment to generate strand specific library product compatible with illumina NGS platform..

Features

  • Simple workflow - most components are supplied as ready-to-use master mixtures which reduces setup time and liquid handling steps
  • Great performance - comparable quality with market leading products
  • Wide dynamic range - purified mRNA could be down to 50 ng
  • Automation capable protocol
  • Applications

  • Expression of all coding RNAs
  • Identification of alternative splicing events
  • Detection of single nucleotide polymorphisms or mutations
  • Discretion of translocations and fusion transcripts
  • Discovering of allele specific expression patterns
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