mRNA Sample Prep Kit
Introduction to mRNA Sample Prep Kit
Transcriptome sequencing or RNA-seq is a novel method for gene expression analysis. Advantages of RNA-Seq include no bias toward known RNA molecules as with probe-based technologies, ability to detect novel and rare transcripts or novel alternative splice isoforms, and direct measurement of transcript abundance within biological samples. BioChain's RapidSeq Sample Prep Kits provide high quality solutions to the RNA-Seq Next Generation sequencing research and diagnostic communities. The RapidSeq Directional mRNA Sample Prep kit aims to prepare directional NGS libraries for subsequent cluster generation, using purified mRNA as starting material. Our kit provides strand-specific cDNA synthesis with reduced costs and increased sensitivity. Strand-specific RNA-seq improves on standard RNA-seq in three ways: accurately identifying antisense transcripts, determining the transcribed strand of non-coding RNAs (e.g. lincRNAs), and demarcating the boundaries of closely situated or overlapping genes. The protocol includes steps for mRNA fragmentation, purification, adapter ligation, reverse transcription, PCR amplification, and DNA fragment enrichment to generate strand specific library product compatible with illumina NGS platform..