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Next Generation Sequencing

Next Generation Sequencing

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Next Generation Sequencing

Introduction to Next Generation Sequencing

Special Offer* Buy One Sample Prep Kit - Rapidseq Directional mRNA Sample Prep Kit, Cat# KS073012, KS073012-I - IV, KS075012, KS075012-I - IV - Rapidseq Small RNA Sample Prep Kit, Cat# KS071012, KS071012-I - IV, KS074012, KS074012-I - IV Get One Purification Kit FREE! - Magseq mRNA Purification Kit regular price $299, Cat# K2012008 - MicroRNA Isolation Kit, regular price $199, Cat# KS341025 - Whole Blood RNA Extraction Kit, regular price $259, Cat# K1012050 * Maximum one free item per order. Discount will be applied during check out. Promotion ends 12/31/2012. The currently available massive parallel sequencing is revolutionizing the researches in genomics, life science and medical applications. Next-generation sequencing technologies include a number of methods that are grouped broadly as template preparation, sequencing and imaging, and data analysis. The unique combination of specific protocols distinguishes one technology from another and determines the type of data produced from each platform. However, the basic sample process requirements, so called library construction, are shared among all of the technology platforms. BioChain offers microRNA and mRNA sample library construction kits to allow fast and cost-effective NGS sequencing. Out kits have the following advantages: (1) streamlined workflow process; (2) reduced cost of reagents; (3) easy to use, all-in-one kit. With these features, our kit enhances productivity by shortening the time required for library preparation and decreases the potential human error rate since we reduced the hands-on time for sample preparation.
Features Low RNA input required Up to 48 barcodes available for multiplexing Simplified workflow reduces hands-on time Flexible barcode options In MasterMix and High Yield formats
mRNA Smaple Prep Kit The RapidSeq Directional mRNA Sample Prep kit provides strand-specific cDNA synthesis with reduced costs and increased sensitivity. Strand-specific RNA-seq improves on standard RNA-seq in three ways: accurately identifying antisense transcripts, determining the transcribed strand of non-coding RNAs (e.g. lincRNAs), and demarcating the boundaries of closely situated or overlapping genes. • View All mRNA Sample Prep Kits

Small RNA Sample Prep Kit Developed to provide the most cost-effective solution to the RNA-seq next gen sequencing research and diagnostic community, this kit requires as little as 100 pg total RNA which makes the whole genome microRNA analysis in many rare sources possible • View All Small RNA Sample Prep Kits	mRNA Purification Kit MagSeq mRNA Purification Kit contains reagents, including oligo (dT) magnetic beads, which is enough for 8 mRNA isolation. The kit’s set up is perfectly compatible for next generation sequencing instrument. • View mRNA Purification Kit

Sample Prep Enzymes Enzymatic engineered T4 RNA Ligase 1 and truncated T4 RNA Ligase 2 increase the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth meanwhile minimize the primer dimer band contamination. High efficient and thermostable M-MLV Reverse Transcriptase ensures unbiased cDNA synthesis for all RNA species. • View All Sample Prep Enzymes	MicroRNA Isolation Kit microRNA Isolation Kit provides a rapid method for the isolation and purification of small RNA molecules (<200 nt) from tissue culture cells and small tissues. This kit can also be used for isolating large RNA (>200 nt). This kit contains enough reagents for 25 isolations of small RNAs (<200 nt) or 50 isolations of large RNAs (>200 nt). • View microRNA Isolation Kit

DNA Storage Solution DNA Storage Solution is used for dissolving DNA. Unlike the general TE buffer, this solution can keep DNA concentration more stable, especially for low concentration DNA. This solution is especially good for storing DNA for next generation sequencing purpose, helps to avoid decreasing the cluster numbers due to repeated freeze and thaw cycles. • View DNA Storage Solution