Sample Prep Enzymes
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T4 RNA Ligase 1 catalyzes the ligation of a 5' phosphorylated nucleic acid donor to a 3' hydroxyl nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates
T4 RNA Ligase 2, truncated specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA. The enzyme does not require ATP for ligation but does need the pre-adenylated substrate. The enzyme is expressed from a plasmid in E. coli which encodes the first 249 amino acids of the full length T4 RNA Ligase 2. This enzyme has been used for optimized linker ligation for the cloning of microRNAs. This enzyme reduces background ligation because it can only use adenylated primers.
- Minimized RNaseH activity
- Long RNA template reverse transcription ability (>5kb)
- Reaction temperature range between 42 oC -50 oC
Engineered and purified with cutting edge technology, M-MLV RT ensures long fragment (up to 5kb), high fidelity, high yield in cDNA synthesis when used in gene expression profiling and RNA sample prep for NGS.
- Thermostable Activity
- Enhanced Performance
UltraScript RT is proprietary engineered and FPLC purified high performance molecular biology tool for the toughest tasks in cDNA library construction, gene expression studies and next generation sequencing. With its high thermal stability, high specificity and more full length cDNA synthesis, the optimal fist-strand cDNA synthesis temperature for this enzyme is 60oC, and it has a broad working temperature range from 37oC to 65oC, with cDNA product size from 100 bp to 12 Kb.
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