Give Your Sample Prep a Boost! Introducing Biochain UltraScriptTM Reverse Transcriptase and M-MLV Reverse Transcriptase.
UltraScriptTM Reverse Transcriptase
Figure 1. Secondary structure of mRNA 5' UTR as the main hindrance of reverse transcription.
|Figure 2. PCR amplification products comparison followed by reverse transcription using 5 μg, 100 ng, 1 ng human total RNA respectively in a 20 μl reaction solution containing 200 units Ultrascript RT (BioChain) or Supplier I RT
|Figure 3. Comparison of UltraScript RT and Supplier I RT on a 12kb human Titin ORF. qPCR was performed to quantify the synthesized first strand Titin cDNA using primers targeted at a specific region (11561‐11737 bp) of reverse transcribed Titin cDNA.
M-MLV Reverse Transcriptase
Figure 4. Reverse transcription with 100 ng human placenta RNA in a 20 µl reaction solution containing 200 units M-MLV RTase. 1 µl reverse transcription solution was used as template for a 25 µl PCR solution with primers for three different abundance genes at 42 °C.
β-2-microglobulin (B2M) (expression level 9000 AU)
Transcription factor 7-like 2 (TCF7L2) expression level 200 AU)
Tubulin (TUBB1) (expression level <10AU)