United States

Reverse Transcriptase

Have a question or just need help?
Contact us for Service and Support

Give Your Sample Prep a Boost! Introducing Biochain UltraScriptTM Reverse Transcriptase and M-MLV Reverse Transcriptase.
 

UltraScriptTM Reverse Transcriptase

- Cat # L4121050, L4121100

  • Thermostable Activity ‐ performs reverse transcription throughout a wide range of 42°C ~ 65°C
  • Enhanced Performance – handles high and low RNA concentrations, short and long RT target sizes
  • Reproducibility ‐ strict controlled production/purification ensures reproducible results
  • Stability ‐ stable at room temperature for a month, one year at 4°C and for 2 years in a ‐20°C freezer
  •  

    RTases

    Figure 1. Secondary structure of mRNA 5' UTR as the main hindrance of reverse transcription.
    UltraScript Figure 2. PCR amplification products comparison followed by reverse transcription using 5 μg, 100 ng, 1 ng human total RNA respectively in a 20 μl reaction solution containing 200 units Ultrascript RT (BioChain) or Supplier I RT
     
    Figure 3. Comparison of UltraScript RT and Supplier I RT on a 12kb human Titin ORF. qPCR was performed to quantify the synthesized first strand Titin cDNA using primers targeted at a specific region (11561‐11737 bp) of reverse transcribed Titin cDNA.
     

     

    M-MLV Reverse Transcriptase

    Cat # K5040002, K5040002-100K

  • Minimized RNaseH activity
  • Long RNA template reverse transcription ability (>5kb)
  • Reaction temperature ranges between 42 °C -50 °C
  •  

     

    Figure 4. Reverse transcription with 100 ng human placenta RNA in a 20 µl reaction solution containing 200 units M-MLV RTase. 1 µl reverse transcription solution was used as template for a 25 µl PCR solution with primers for three different abundance genes at 42 °C.

    β-2-microglobulin (B2M) (expression level 9000 AU)

    Transcription factor 7-like 2 (TCF7L2) expression level 200 AU)

    Tubulin (TUBB1) (expression level <10AU)