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Protein Extraction Systems

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Biochain Protein Extraction System


BioChain’s kits quickly extract either total proteins from your tissues or cells, or enrich for subcelluar fractions. We make it easy to study what matters most, whether you’re interested in total proteins (K3011010), membrane proteins (K3014005), mitochondrial proteins (KC010100) or proteins from specific subcellular fractions (K3013010).


Protein Extraction System

 

 

Catalog#      Product Name


K3011010      Total Protein Extraction Kit

K3013010      Subcellular (CNMCS) Protein Extraction Kit (Cytoplasmic, Nuclear, Membrane, and CytoSkeletal Proteins)

KC010100     Mitochondria Isolation Kit

K3014005      Membrane Protein Extraction Kit

Applications


  • Total Protein Extraction
  • Membrane Protein Extraction
  • Subcellular Protein Extraction
  • Mitochondria Isolation

  • Quality Control


    Proteins extracted from rat colon tissue using Biochain’s Total Protein Extraction Kit and Subcellular (CNMCS) Protein Extraction Kit were submitted to SDS-PAGE in five identical gels. These gels were either Coomassie stained or immunoblotted with antibodies raised against proteins known to be localized in specific subcellular compartments. 


      

     

    Coomassie stained gel displays distinct protein patterns for the total protein fraction (T), cytoplasmic fraction (CY), nuclear fraction (N), membrane fraction (M), and cytoskeletal fractions (CS).

     

     

     

     


    Immunoblotting of proteins transferred from the four remaining gels to PVDF membranes. The first was incubated with antibodies to cytoplasmic protein GAPDH, the second with antibodies to nuclear protein Histone H1, the third with antibodies to membrane protein Na+/K+ ATPase, and the fourth with antibodies to cytoskeleton marker protein vimentin




    Related Products


    • Human Adult Normal Protein             
    • Human Fetal Normal Protein              
    • Human Diabetes Protein              
    • Human Tumor Protein              
    • Human Tumor Cell Line Protein 
    • Human Heart Disease Protein 
    • Human Lung Disease Protein 
    • Human Liver Cirrhosis Protein 
    • Human Lupus Protein 
    • Human Neurological Disease Protein 
    • Mouse Normal Protein
    • Membrane Protein
    • Universal Protein

     



    Selected Publications with Biochain Products

     

    Nucleophosmin, a Critical Bax Cofactor in Ischemia-Induced Cell Death
    Zhiyong Wang, Jonathan M. Gall, Ramon Bonegio, Andrea Havasi, Katarina Illanes, John H. Schwartz, and Steven C. Borkan
    Mol. Cell. Biol., May 2013; 33: 1916 - 1924.

    A critical role of astrocyte-mediated nuclear factor- B-dependent inflammation in Huntington's disease
    Han-Yun Hsiao, Yu-Chen Chen, Hui-Mei Chen, Pang-Hsien Tu, and Yijuang Chern
    Hum. Mol. Genet., May 2013; 22: 1826 - 1842.

    Proteasome Dysfunction Mediates Obesity-Induced Endoplasmic Reticulum Stress and Insulin Resistance in the Liver
    Toshiki Otoda, Toshinari Takamura, Hirofumi Misu, Tsuguhito Ota, Shigeo Murata, and et al.
    Diabetes, Mar 2013; 62: 811 - 824.

    Galectin-1 Promotes Lung Cancer Progression and Chemoresistance by Upregulating p38 MAPK, ERK, and Cyclooxygenase-2
    Ling-Yen Chung, Shye-Jye Tang, Guang-Huan Sun, Teh-Ying Chou, Tien-Shun Yeh, Sung-Liang Yu, and Kuang-Hui Sun
    Clin. Cancer Res., Aug 2012; 18: 4037 - 4047.

    ARL4A acts with GCC185 to modulate Golgi complex organization
    Yu-Chun Lin, Tsai-Chen Chiang, Yu-Tsan Liu, Yueh-Tso Tsai, Li-Ting Jang, and Fang-Jen S. Lee
    J. Cell Sci., Dec 2011; 124: 4014 - 4026.