PCR, RT-PCR, Real-Time qPCR

    qPCR SuperMix

BioChain’s Supermix kits optimize qPCR with complex environmental conditions. In addition, the Eva Green dye based and TaqMan probe based SuperMix kits ensure the high quality qPCR result by efficiency and reproducibility. BioChain’s qPCR SuperMix kits produce cleaner products with improved melting curves comparing with other competitors.

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    qRT-PCR SuperMix

BioChain’s QCell one-step qRT-PCR SuperMix kits are used to directly amplify RNA of your interest from the cell or tissue samples. We combined the reverse transcription (RT) and qPCR steps as well as the cell/tissue lysis in the same reaction tube. This significantly reduces the number of experimental steps and as the result, diminishes error ratio rooted from the separated procedure. This also enables the high throughput gene expression screening and simple RNA quantification experiments.

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    PCR Mix

BioChain’s PCR master mix provides all ingredients necessary for performing PCR in a premixed and optimized format that streamlines and simplifies the PCR workflow. Master mixes come pre-mixed and pre-measured containing DNA polymerase, salts, magnesium, dNTPs and optimized reaction buffer. Your template and primers are the only additions necessary to perform PCR.

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     Taq Polymerase

BioChain’s chemically-modified Taq hot-start method is licensed from Applied Biosystems, using a Taq polymerase which has been modified with the addition of a heat labile blocking group that inactivates the enzyme at room temperatures. After 95°C incubation for 10 minutes the results in removal of the group and activation of Taq enzyme.    

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     dNTPs

dNTPs (deoxyribonucleotide triphosphates) are critical for PCR, DNA/RNA sequencing and cDNA synthesis. BioChain offers a variety of high quality dNTPs that can help you to achieve optimum PCR results

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     Bacteria Identification Kit

Bacteria identification kit is an ultra-sensitive and universal system developed by Biochain to identify bacteria by PCR. It detects bacteria at concentration of 0.2 or more cfu/per PCR reaction. This kit can detect different types of Gram negative or positive rod and coccus bacteria, such as E. Coli, K. Pneumoniae, B. Cereus and S. Agalactiae, etc.  

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     Primer Pairs

BioChain’s Primer Pairs are designed according to certain key parameters, such as specificity, length, optimal AT to GC
ratio, secondary structure, stability, and Tm. All primers are optimized to multiple PCR reactions under the same PCR reaction conditions. Therefore, PCR reaction with different primer sets can be applied simultaneously on the same instrument or the same tube. The PCR products or sequences of Primer Pairs are confirmed through the size of PCR product, restriction mapping or nested PCR analysis. Every set of primers is tested and quality controlled by verifying these parameters.             • View Primer Pairs