Introduction to MicroRNA MarkersBioChain's microRNA Biomarker Real-time RT-qPCR Detection Kit provides a specific assay for quantitative analysis of miRNA expression from total RNA samples (containing miRNAs) or small RNAs enriched samples. This kit contains all the reagents needed for running the real-time RT-qPCR assay, including reverse transcription reagents and primer, qPCR reaction reagents and specific primer/probe mixture. The proprietary design of nd Guide?primer is to facilitate the binding of the miRNA to our primer adapter. The PCR primers/probe mixture includes a forward primer, a reverse primer and a TaqMan probe dual-labeled with 5'-FAM and BHQ-1-3? which is used for real-time detection and quantification of target cDNA.
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