MicroRNA Markers

Introduction to MicroRNA Markers

BioChain's microRNA Biomarker Real-time RT-qPCR Detection Kit provides a specific assay for quantitative analysis of miRNA expression from total RNA samples (containing miRNAs) or small RNAs enriched samples. This kit contains all the reagents needed for running the real-time RT-qPCR assay, including reverse transcription reagents and primer, qPCR reaction reagents and specific primer/probe mixture. The proprietary design of nd Guide?primer is to facilitate the binding of the miRNA to our primer adapter. The PCR primers/probe mixture includes a forward primer, a reverse primer and a TaqMan probe dual-labeled with 5'-FAM and BHQ-1-3? which is used for real-time detection and quantification of target cDNA.

Features

Convenient - All reaction components are supplied as ready to use mixtures, reducing set up time and liquid handling steps for quick and easy set up

High sensitivity - Detect miRNA expression with both total RNA (containing miRNAs) or small RNA enriched samples

Wide dynamic range - Linearity over 6 logs of dynamic range

Flexible - Compatible with various real-time qPCR instruments and different qPCR formats

Applications

MicroRNA biomarker detection and quantification

MicroRNA expression profiling

MicroRNA array data validation

Reference

1. Hiroshi Hirata, Yuji Hinoda, Koji Ueno, Varahram Shahryari, Z.Laura Tabatabai, and Rajvir Dahiya MicroRNA-1826 targets VEGFC, beta-catenin (CTNNB1) and MEK1 (MAP2K1) in human bladder cancer Carcinogenesis, Jan 2012; 33: 41 - 48. Full text

2. Feng B, Chen S, McArthur K, Wu Y, Sen S, Ding Q, Feldman RD, Chakrabarti S. miR-146a-Mediated extracellular matrix protein production in chronic diabetes complications. Diabetes. 2011 Nov;60(11):2975-84. Full text