Total RNA

Introduction to Total RNA

BioChain's Total RNAs are isolated from a variety of human adult and fetal normal tissues, human diseased and tumor tissues, as well as mouse, rat, and monkey tissues. The Total RNAs are provided ready-to-use for Northern blotting, cDNA synthesis, RNA protection, microRNA study, and RNA differential display. This product line saves researchers the time of RNA sample preparation and enables immediate study of genes of interest in many different tissues.

Features

Total RNA isolated from a wide variety of hard to obtain tissues

Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA

Extensive quality control procedures to ensure high quality

High efficiency reverse transcription

MicroRNA certified

Available documentation of tissues' clinical histories

Applications

Northern Blotting, RT-PCR, and RACE

cDNA synthesis and cDNA library construction

cDNA probe for profiling study in gene expression

RNA protection and primer extension

RNA display

Purification of mRNA

MicroRNA study

Quality Control

The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-HCl, pH 7.5).

The RNA is treated by DNAse I, and is tested as DNA free RNA by PCR.

cDNA synthesis is successfully performed by using this RNA as template.