mRNA

Introduction to mRNA

BioChain's mRNA is series of products isolated from total RNA extracted from a variety of human adult and fetal normal tissues, human tumor and diseased tissues, as well as mouse, rat, monkey, and plant tissues. The RNA undergoes two rounds of oligo(dT) selection utilizing BioChain's proprietary technology to minimize the presence of ribosomal RNA. Potential DNA contaminants are removed by a stringent DNase treatment. The purity and intactness of the mRNA is verified by agarose gel electrophoresis, Northern analysis, cDNA synthesis, and capillary electrophoresis using an Agilent 2100 BioAnalyzer.

Features

mRNA isolated from a wide variety of tissues and cells

DNase I treatment to ensure removal of genomic DNA

RNA intactness (lengths reach over 12 kb)

High efficiency reverse transcription

Representative of total RNA

Documentation of tissues' clinical histories available

Applications

Northern Blotting, RT-PCR, and RACE

cDNA synthesis and cDNA library construction

cDNA probe for gene expression profiling

RNA protection and primer extension

RNA display and reverse Northern blotting

In vitro translation