Introduction to cDNA
BioChain's PCR ready first strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. We carry the largest selection of tissue cDNAs on the research market, covering over 358 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues
Features Ready to use for PCR
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12 kb PCR amplicon was obtained to ensure the intact cDNAs
The largest selection of cDNAs from different tissues on the market
Documentation of tissues' clinical histories available
Applications Immediate PCR Amplification of known genes
Verification of genetic mutation
Comparison of a specific gene between different tissues
Analysis of mRNA alternative splicing
Gene cloning and target sequencing
The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.
The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
The synthesized human, animal, and cell line cDNA was selected to ensure its full length. The cDNA was used as template for PCR amplification of ß-actin gene and an 838 bp ß-actin band was visualized on 1% agarose gel. ß-actin control primer is included. It is enough for 10 PCR reactions.
The synthesized plant cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions.