Introduction to cDNA Panel
BioChain's cDNA panel is comprised of 5 tubes each containing 10 µl of PCR Ready First Strand cDNAs from 5 tissues. This is the economical way to get cDNA from multiple tissues. These cDNAs are ideal for analyzing gene expression patterns and discovering novel genes. All cDNAs in a panel are made under optimal and consistent conditions, which ensure that the genetic representation is maintained from total RNA to cDNA.
Features Ready to use for PCR quantification of gene expression
Oligo dT primer used to ensure the entire 3' end of cDNA is present
With some cDNA used as templates, 12 kb PCR amplicon was obtained to ensure intact cDNAs
The largest selection of cDNAs from different tissues on the market
Placenta cDNA is included in every panel as an interpanel control
Documentation of tissues' clinical histories available
Applications PCR amplification of known genes for expression
Quantification of low copy gene expression in multiple tissues
Identification of tissue-specific expression of target genes
Analysis of gene expression in rare tissues
Verification of genetic mutation
Comparison of a specific gene between different tissues
Analysis of mRNA alternative splicing
The integrity of the RNA used for cDNA synthesis is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel. The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). The ratio of 28S/18S is ≥1.
The RNA used for cDNA synthesis is treated by DNase I, and is tested as DNA free RNA by PCR.
The synthesized human, animal, and cell line cDNA was selected to ensure its full length. The cDNA was used as template for PCR amplification of ß-actin gene and an 838 bp ß-actin band was visualized on 1% agarose gel. ß-actin control primer is included. It is enough for 10 PCR reactions.
The synthesized plant cDNA was used as template for PCR amplification of chloroplast gene. A 458 bp chloroplast band was visualized on 1% agarose gel. Chloroplast control primer is included. It is enough for 10 PCR reactions.