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Biospecimen DNA, Purification & Analysis

Biospecimen DNA, Purification & Analysis

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Biospecimen DNA, Purification & Analysis

Introduction to Biospecimen DNA, Purification & Analysis

BioChain provides a spectrum of DNA products derived from various organ systems and tissues of the human body with different developmental stage and disease conditions. DNA materials extracted from different species of plants and animals are also available. Our DNAs derived from hard-to-find diseased tissues, such as the matched pairs of tumor tissue (both the tumor with matching adjacent normal tissues and the primary tumor with metastatic tumor tissues), are invaluable tools for discovery research and diagnostic product development. In addition to the biospecimen DNA materials, we also offer DNA extraction, purification, and analysis reagents and kits for our customers to perform the tasks on their own samples.

96 Well Genomic DNA Plate

Designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA) from whole blood and a variety of normal or diseased tissues or cells. They are intended for human normal and tumor gene profiling studies in automated gene analysis systems.

• Human Normal Genomic DNA Plate
• Human Breast Tumor Genomic DNA Plate
• Human Colon Tumor Genomic DNA Plate
• Human Lung Tumor Genomic DNA Plate
• Human Stomach Tumor Genomic DNA Plate

cDNA Library

The cDNA libraries provided by BioChain are subjected to rigorous quality control testing that ensures the quality with 106 minimum numbers of primary clones, 3x106 average numbers of primary clones and minimum 87% of recombinant coverage and 95% average of recombinant coverage.

• Human Adult Normal cDNA Library
• Human Fetal Normal cDNA Library
• Monkey (Rhesus) Normal cDNA Library
• Mouse Normal cDNA Library
• Xenopus Normal cDNA Library

Universal cDNA

Universal cDNA is prepared by reverse transcription from normal human tissues RNA using random hexamer or oligo dT primers. Total RNA is isolated using the modified guanidine thiocyanate techniques.

• Human Universal cDNA
• Monkey Universal cDNA
• Mouse Universal cDNA
• Rat Universal cDNA
• Dog Universal cDNA
• Chicken Universal cDNA

PCR Ready cDNA

BioChain's PCR ready first strand cDNA is tissue specific and are ready-to-use for gene discovery or expression analysis. We carry the largest selection of tissue cDNAs on the research market, covering over 358 cDNAs from human adult and fetal normal tissues, human diseased and tumor tissues, rat, mouse, monkey and plant tissues.

• Human Adult Normal cDNA
• Human Fetal Normal cDNA
• Human Diabetes cDNA
• Human Heart Disease cDNA
• Human Lung Disease cDNA
• Human Neurological Disease cDNA
• Human Tumor cDNA
• Human Tumor Cell Line cDNA
• Human Liver Cirrhosis cDNA
• Monkey (Cynomolgus) Normal cDNA
• Monkey (Rhesus) Normal cDNA
• Mouse Normal cDNA
• Nude Mouse Normal cDNA
• Rat Normal cDNA
• Dog Normal cDNA
• Bovine Normal cDNA
• Porcine Normal cDNA
• Guinea Pig Normal cDNA
• Hamster Normal cDNA
• Rabbit Normal cDNA
• Plant Normal cDNA

Dr. P (DNA, RNA, Protein) - Set

BioChain's Dr. P product includes genomic DNA, RNA, and protein isolated simultaneously from a single biomaterial source, such as tissues or cells; hence, the name. The Dr.P set is available from a wide variety of tissues, and is good for the simultaneous study of RNA, genomic DNA, and proteins.

• Dr. P Set - Human Adult Normal Tissues
• Dr. P Set - Human Fetal Normal Tissues
• Dr. P Set - Human Tumor Tissues
• Dr. P Set - Monkey (Cynomolgus) Normal Tissues
• Dr. P Set - Monkey (Rhesus) Normal Tissues
• Dr. P Set - Mouse Normal Tissues
• Dr. P Set - Rat Normal Tissues

Genomic DNA

BioChain's genomic DNAs are isolated from over 100 different human and fetal normal tissues, human diseased and tumor tissues, mouse, rat, monkey, and plant tissues. Our genomic DNAs are provided ready to use for SNP analysis, DNA methylation analysis, copy number variation (CNV) or comparative genomic hybridization (CGH) analysis, Southern Blotting, and PCR. BioChain offers genomic DNAs from almost any tissues of human, mouse, rat, monkey, other animals, and plants. Getting genomic DNAs from BioChain will save you valuable research time to immediately identify genes of interest; no longer will you need to acquire tissues and isolate genomic DNA by yourself.

Small Size Genomic DNA

BioChain's small size genomic DNAs are isolated from a wide variety of tissues by a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNAs are tested by spectrophotometer and electrophoresis. These small size genomic DNAs can be used as PCR template and have shown better performance in digital PCR when compared to the common large size genomic DNAs. A260/280 is between 1.8 and 2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.

• Small Size Human Adult Normal Genomic DNA
• Small Size Human Fetal Normal Genomic DNA
• Small Size Human Diabetes Genomic DNA
• Small Size Human Tumor Genomic DNA
• Small Size Human Tumor Cell Line Genomic DNA
• Small Size Monkey (Cynomolgus) Normal Genomic DNA
• Small Size Monkey (Rhesus) Normal Genomic DNA
• Small Size Mouse Normal Genomic DNA
• Small Size Rat Normal Genomic DNA
• Small Size Plant Normal Genomic DNA

cDNA Panel

BioChain's cDNA panel is comprised of 5 tubes each containing 10 µl of PCR Ready First Strand cDNAs from 5 tissues. This is the economical way to get cDNA from multiple tissues. These cDNAs are ideal for analyzing gene expression patterns and discovering novel genes. All cDNAs in a panel are made under optimal and consistent conditions, which ensure that the genetic representation is maintained from total RNA to cDNA.

• Human Adult Normal cDNA Panel
• Human Disease cDNA Panel
• Human Tumor Cell Line cDNA Panel
• Human Tumor Different Type Different Donor cDNA Panel
• Human Tumor Same Type Different Donor cDNA Panel
• Monkey (Rhesus) Normal cDNA Panel
• Monkey (Cynomolgus) Normal cDNA Panel
• Mouse Normal cDNA Panel
• Rat Normal cDNA Panel

DNA Analysis

We provide a series of popularly used reagents and kits that enabled our customers to easily analyze genomic DNA, cDNA, and plasmid DNA.

• cDNA Synthesis Kit
• DNA Loading Buffer
• DNA Methylation Detection Kit
• Express Cloning Checker Kits

DNA Extraction

We provide convenient tool for isolating high quality genomic DNA. The isolated DNA can be used for PCR amplification template, Southern Blot analysis, SNP analysis, and DNA methylation research. Each kit can be used to isolate genomic DNA from 5 grams of tissue and cells.

• Blood and Serum DNA Isolation Kit
• Genomic DNA Extraction Kit
• EZ-Blood DNA 96 Kit
• EZ DNA 96 Kit (Cells and Tissues)
• FFPE Tissue DNA Extraction Kit
• GoldMag Whole Blood Genomic DNA Purification Kit
• Saliva DNA Isolation Kit
• Serum DNA Isolation Kit
• Urine DNA Isolation Kit
• EasyXpress Viral Nucleic Acid Release Kit

DNA Markers

Identification of DNA biomarkers holds promise for diagnosis and monitoring of disease progression. We provide a number of cancer related DNA marker for such applications.

• Human Breast Cancer DNA Markers
• Human Colorectal Cancer DNA Marker

Dr. P (DNA, RNA, Protein) Kit

Dr. P takes its name from genomic DNA, RNA, and Protein isolated simultaneously from a single biomaterial source, such as tissue or cells. BioChain not only provide Dr. P products from a wide variety of tissues but also provides Dr. P Kit. With this kit, scientists are able to isolate genomic DNA, RNA, and protein from their own biomaterials.

• Dr. P Kit

Matched Pair - cDNA

BioChain's human cDNA matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of cDNAs isolated from primary tumor and its adjacent normal tissue; PM consists of cDNAs from primary tumor and corresponding metastatic tumor. cDNAs in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic genes.

• Matched Pair – Human Tumor cDNAs

Matched Pair – Genomic DNA

BioChain's human genomic DNA matched pair products include: Primary Pair (PP), Primary and Metastatic Pair (PM). PP consists of genomic DNAs isolated from primary tumor and its adjacent normal tissue; PM consists of genomic DNAs from primary tumor and corresponding metastatic tumor. Genomic DNAs in each pair are prepared from the same donor. This product line is designed for identifying tumor-specific genes and tumor metastatic genes.

• Matched Pair – Human Tumor Genomic DNAs

Molecular Weight Markers

BioChain provides a series of molecular weight markers, including DNA markers, RNA markers, and protein markers, to accurately determine the size of your DNA, RNA, and protein on the separation gels.

• DNA Markers
• RNA Markers
• Protein Markers

Human Stem Cell cDNA Library

BioChain's Human Embryonic Stem Cell and Stem Cell Line cDNA libraries are constructed by using an oligo dT primer-adapter and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) to prime and synthesize first strand cDNA from mRNA. After the second strand is synthesized, the double stranded cDNA is size fractionated, cloned directionally into our special vectors and transformed into T1 phage resistant E. coli. We also provide both standard and normalized cDNA libraries.

• Human Stem Cell cDNA Library