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MicroRNA Markers

Introduction to MicroRNA Markers

BioChain's microRNA Biomarker Real-time RT-qPCR Detection Kit provides a specific assay for quantitative analysis of miRNA expression from total RNA samples (containing miRNAs) or small RNAs enriched samples. This kit contains all the reagents needed for running the real-time RT-qPCR assay, including reverse transcription reagents and primer, qPCR reaction reagents and specific primer/probe mixture. The proprietary design of nd Guide?primer is to facilitate the binding of the miRNA to our primer adapter. The PCR primers/probe mixture includes a forward primer, a reverse primer and a TaqMan probe dual-labeled with 5'-FAM and BHQ-1-3? which is used for real-time detection and quantification of target cDNA. * For product availability, please email us at info@biochain.com, submit your question online at Contact Us or call us at 1-888-762-2568

Features

  • Convenient - All reaction components are supplied as ready to use mixtures, reducing set up time and liquid handling steps for quick and easy set up
  • High sensitivity - Detect miRNA expression with both total RNA (containing miRNAs) or small RNA enriched samples
  • Wide dynamic range - Linearity over 6 logs of dynamic range
  • Flexible - Compatible with various real-time qPCR instruments and different qPCR formats
  • Applications

  • MicroRNA biomarker detection and quantification
  • MicroRNA expression profiling
  • MicroRNA array data validation
  • Reference

    1. Hiroshi Hirata, Yuji Hinoda, Koji Ueno, Varahram Shahryari, Z.Laura Tabatabai, and Rajvir Dahiya MicroRNA-1826 targets VEGFC, beta-catenin (CTNNB1) and MEK1 (MAP2K1) in human bladder cancer Carcinogenesis, Jan 2012; 33: 41 - 48. Full text

    2. Feng B, Chen S, McArthur K, Wu Y, Sen S, Ding Q, Feldman RD, Chakrabarti S. miR-146a-Mediated extracellular matrix protein production in chronic diabetes complications. Diabetes. 2011 Nov;60(11):2975-84. Full text