United States

EZ-RNA 96 Kit(Cells and Tissues)

Introduction to EZ-RNA 96 Kit(Cells and Tissues)

Biochain's EZ-RNA 96 Kit is designed for the high-throughput purification of total cellular RNA from cultured cells in a 96-well plate format. No phenol-chloroform extraction or precipitation steps are involved. The kit can also be used to cleanup up to 96 RNA samples at a time. The sample addition and washing steps are performed using any 96-well compatible vacuum manifold, while the final elution of the RNA product is performed using a table-top centrifuge. Cells are lysed under denaturing conditions using a lysis buffer containing the denaturant guanidine isothiocyanate. Ethanol is next added to the samples, which are then added to the filter plates. This step facilitates the binding of RNA to the filter plate's silica membrane matrix. Under these conditions the RNA binds to the membrane while other contaminants are washed through. The plate is then washed to further remove protein, buffer components and other contaminants using two ethanol-containing wash buffers and the final RNA product is elutd in RNase-free water.

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