United States


mRNA

Introduction to mRNA

BioChain's mRNA is series of products isolated from total RNA extracted from a variety of human adult and fetal normal tissues, human tumor and diseased tissues, as well as mouse, rat, monkey, and plant tissues. The RNA undergoes two rounds of oligo(dT) selection utilizing BioChain's proprietary technology to minimize the presence of ribosomal RNA. Potential DNA contaminants are removed by a stringent DNase treatment. The purity and intactness of the mRNA is verified by agarose gel electrophoresis, Northern analysis, cDNA synthesis, and capillary electrophoresis using an Agilent 2100 BioAnalyzer.

Features

  • mRNA isolated from a wide variety of tissues and cells
  • DNase I treatment to ensure removal of genomic DNA
  • RNA intactness (lengths reach over 12 kb)
  • High efficiency reverse transcription
  • Representative of total RNA
  • Documentation of tissues' clinical histories available
  • Applications

  • Northern Blotting, RT-PCR, and RACE
  • cDNA synthesis and cDNA library construction
  • cDNA probe for gene expression profiling
  • RNA protection and primer extension
  • RNA display and reverse Northern blotting
  • In vitro translation
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