Matched Pair - Total RNA
Introduction to Matched Pair - Total RNA
BioChain’s Matched Pair Total RNAs have been generated from human tumors and the corresponding normal tissues from the same donor. BioChain offers Primary Pair (PP), as well as Primary and Metastatic Pair (PM) products. Primary Pair Total RNAs are those that have been isolated from primary tumors and its adjacent unaffected normal tissue. Primary Metastatic Pair Total RNAs have been isolated from primary tumors and corresponding metastatic tumors. Total RNA is isolated by modified guanidine thiocyanate techniques and stored in RNA storage buffer. This product is a useful tool for accelerating gene expression analysis by identifying tumor-specific genes and tumor metastatic genes.
FeaturesTotal RNA isolated from tumor and normal tissues from the same patient
Decontamination of polysaccharide, proteoglycan, RNase, and genomic DNA
Extensive quality control procedures to ensure high quality
High efficiency reverse transcription
Documentation of tissues' clinical history is available
ApplicationsNorthern Blotting, RT-PCR, and RACE
cDNA synthesis and cDNA library construction
cDNA probe for profiling study in gene expression
RNA protection and primer extension
Purification of mRNA
Next Generation RNA Sequencing
The integrity of the RNA is examined by visual inspection for the presence of intact bands of 18s and 28s ribosomal RNA when electrophoreses on a denaturing agarose gel
The quality and purity of total RNA were tested by spectrophotometer. A260/280 is between 1.8 and 2.1(detected in 10 mM Tris-HCl, pH 7.5).