United States


CNMCS Compartmental (Subcellular) Protein Extraction Kit (Tissue and Cells)

6 Item(s)

per page
CATALOG #LOT#PRODUCT NAMESIZEPRICESTOCKBUY
K3013010
CNMCS Compartmental Protein Extraction Kit 1 kit $429

In Stock


K3013010-1
CNMCS Compartmental Protein Extraction Kit: Buffer C 18 ml $63

In Stock


K3013010-5
CNMCS Compartmental Protein Extraction Kit: Buffer CS 3 ml $63

In Stock


K3013010-4
CNMCS Compartmental Protein Extraction Kit: Buffer M 6 ml $63

In Stock


K3013010-3
CNMCS Compartmental Protein Extraction Kit: Buffer N 6 ml $63

In Stock


K3013010-2
CNMCS Compartmental Protein Extraction Kit: Buffer W 50 ml $63

In Stock


6 Item(s)

per page

Introduction to CNMCS Compartmental (Subcellular) Protein Extraction Kit (Tissue and Cells)

BioChain’s compartmental (subcellular) protein extraction kit is an innovative, easy-to-perform, and cost-effective method to sequentially isolating cytoplasmic, nuclear, membrane, and cytoskeletal proteins from tissues and cells. This kit is provided with protease inhibitor cocktail to prevent protein degradation and five reagents which is enough to enrich four compartmental proteins from 5 grams of tissue or cells. The efficiency of subcellular fractionation has been investigated by SDS-PAGE and immunoblotting of selected marker proteins.

Features

  • Provides all components for stepwise preparation of proteins from tissues and cells
  • Isolate four compartmental proteins in less than three hours
  • Increase detection sensitivity by enriching a subset of proteins of interest
  • Easy to use compared to other methods such as differential centrifugations
  • Applications

  • Prepare proteins for downstream applications such as SDS-PAGE, Western blotting, gel mobility shift, protein assays, and other procedures
  • Detection of differential post-translational modifications or differential subcellular localization of target proteins
  • Enrichment of low-abundance proteins for visualization and subsequent analysis
  • Use to study: DNA binding proteins such as transcription factors, soluble proteins abundant in cytosol, membrane proteins such as receptors, and cytoskeletal proteins
  • Documents

    Reference

    1. Hsieh TH, Tsai CF, Hsu CY, Kuo PL, Lee JN, Chai CY, Hou MF, Chang CC, Long CY, Ko YC, Tsai EM. Phthalates stimulate the epithelial to mesenchymal transition through an HDAC6-dependent mechanism in human breast epithelial stem cells. Toxicol Sci. 2012 Aug;128(2):365-76. <<Abstract >>

    2. LY, Tang SJ, Sun GH, Chou TY, Yeh TS, Yu SL, Sun KH. Galectin-1 promotes lung cancer progression and chemoresistance by upregulating p38 MAPK, ERK, and cyclooxygenase-2. Clin Cancer Res. 2012 Aug 1;18(15):4037-47. <<Abstract >>

    3. Hsieh TH, Tsai CF, Hsu CY, Kuo PL, Lee JN, Chai CY, Wang SC, Tsai EM. Phthalates induce proliferation and invasiveness of estrogen receptor-negative breast cancer through the AhR/HDAC6/c-Myc signaling pathway. FASEB J. 2012 Feb;26(2):778-87. Full text